Europium in PDB 1wc4: Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium

All present enzymatic activity of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium:
4.6.1.1;

The structure of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium, PDB code: 1wc4 was solved by C.Steegborn, T.N.Litvin, L.R.Levin, J.Buck, H.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.91 / 3.0
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.601, 70.202, 99.654, 90.00, 90.00, 90.00
R / Rfree (%)	27.4 / 31.5

The binding sites of Europium atom in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium (pdb code 1wc4). This binding sites where shown within 5.0 Angstroms radius around Europium atom.
In total 2 binding sites of Europium where determined in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium, PDB code: 1wc4:
Jump to Europium binding site number: 1; 2;

Europium binding site 1 out of 2 in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium


Mono view


Stereo pair view

A full contact list of Europium with other atoms in the Eu binding site number 1 of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium within 5.0Å range: probe atom residue distance (Å) B Occ A:Eu2100 b:10.9 occ:1.00 OD1 A:ASP1061 2.3 10.9 1.0 O1B A:APC1500 2.4 10.9 1.0 OD1 A:ASP1017 2.5 10.9 1.0 O A:ILE1018 2.8 10.9 1.0 OD2 A:ASP1061 2.8 10.9 1.0 O1A A:APC1500 2.9 10.9 1.0 CG A:ASP1061 2.9 10.9 1.0 OD2 A:ASP1017 3.0 10.9 1.0 CG A:ASP1017 3.1 10.9 1.0 PB A:APC1500 3.4 10.9 1.0 C3A A:APC1500 3.7 10.9 1.0 O3G A:APC1500 3.7 10.9 1.0 PA A:APC1500 3.9 10.9 1.0 O3B A:APC1500 3.9 10.9 1.0 C A:ILE1018 4.0 10.9 1.0 N A:ILE1018 4.4 10.9 1.0 CB A:ASP1061 4.4 10.9 1.0 PG A:APC1500 4.4 10.9 1.0 O A:HOH2001 4.5 10.9 1.0 CB A:ASP1017 4.5 10.9 1.0 O2A A:APC1500 4.6 10.9 1.0 O2B A:APC1500 4.8 10.9 1.0 CA A:ILE1018 4.8 10.9 1.0 CG2 A:VAL1019 4.9 10.9 1.0 N A:VAL1019 5.0 10.9 1.0

Europium binding site 2 out of 2 in the Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium


Mono view


Stereo pair view

A full contact list of Europium with other atoms in the Eu binding site number 2 of Soluble Adenylyl Cyclase Cyac From S. Platensis in Complex with Alpha,Beta-Methylene-Atp and Europium within 5.0Å range: probe atom residue distance (Å) B Occ B:Eu2100 b:10.9 occ:1.00 OD1 B:ASP1017 2.0 10.9 1.0 O1B B:APC1500 2.1 10.9 1.0 OD1 B:ASP1061 2.3 10.9 1.0 O B:ILE1018 2.6 10.9 1.0 O3G B:APC1500 2.6 10.9 1.0 OD2 B:ASP1061 2.7 10.9 1.0 CG B:ASP1017 2.8 10.9 1.0 CG B:ASP1061 2.9 10.9 1.0 OD2 B:ASP1017 2.9 10.9 1.0 PB B:APC1500 3.2 10.9 1.0 O1A B:APC1500 3.3 10.9 1.0 C3A B:APC1500 3.4 10.9 1.0 C B:ILE1018 3.8 10.9 1.0 PG B:APC1500 3.8 10.9 1.0 O3B B:APC1500 3.8 10.9 1.0 PA B:APC1500 4.0 10.9 1.0 N B:ILE1018 4.1 10.9 1.0 O B:HOH2001 4.2 10.9 1.0 CB B:ASP1017 4.3 10.9 1.0 CB B:ASP1061 4.4 10.9 1.0 O2B B:APC1500 4.5 10.9 1.0 CA B:ILE1018 4.5 10.9 1.0 O1G B:APC1500 4.6 10.9 1.0 N B:VAL1019 4.8 10.9 1.0 CG1 B:ILE1018 4.9 10.9 1.0 C B:ASP1017 4.9 10.9 1.0 O2A B:APC1500 4.9 10.9 1.0 O2G B:APC1500 5.0 10.9 1.0

C.Steegborn, T.N.Litvin, L.R.Levin, J.Buck, H.Wu. Bicarbonate Activation of Adenylyl Cyclase Via Promotion of Catalytic Active Site Closure and Metal Recruitment Nat.Struct.Mol.Biol. V. 12 32 2005.
ISSN: ISSN 1545-9993
PubMed: 15619637
DOI: 10.1038/NSMB880